This study reports the uevelopment and characteristics of Giza 2000. a supenor new barley variety for the newly reclaimed lands and rainfed areas at the North Coast region. It was produced from a cross between the local variety Giza 121 and the Line 388/13/1 which was made in 1978/1979 season. In developing this variety, the check cultivars Giza 123, Giza 124, Giza 125 and Giza 128 were compared with Giza 2000 and two promising lines as well as the regional check Rihane-03. For this purpose, 39 yield trials were conducted from 1998/1999 to 200112002 growing season under rainred conditions as well as under newly reclaimed ands. The average yield of the new cullivar Giza 2000 recorded 3.51 ardab/feddan and ignificantly out yielded Giza 126 under rainled conditions with an average increase of 0.57 rdab/feddan, i.e. 19.39%. In the newly reclaimed lands, the average yield of the new cultivar Giza 2000 significanlly exceeded the average of nalional check Giza 123 by 2.46 ardab/feddan 7.25%). The new variety combines the good characteristics of its parents which including high ielding ability, early maturity, moderate resistance to leaf rust and resistance to powdery mildew and net blotch. Molecular fingerprinting utilizing PCR with 38 random 10mer primers was done for the new variety in order to be used as an appraisal when commercializing this variety and preserve the breeder's rights. A number of 115 reproducible and repealable bands were recovered during PCR. Usefulness of this environment-independent molecular approach was discussed. Seed multiplication of Giza 2000 was started since 2000/2001 growing season to produce certified seeds which will be available to cover the barley production area in Egypt.