The field experiment was carried out in the spring season of 2019 in the Research Station at the College of Agriculture, University of Anbar.This experiment was aimed to study the effect of spraying different concentrations of Brassinolide (0, 0.5, 1, 1.5 mg L^-1) on the yield and quality of three vareities of sunflower (Ishaqi 1, Akmaar, and Sakha). The experimental design was split-plots experiment under the Randomized Completely Block Design (RCBD) with three replicates. The three vareities were distributed in the main-plots and the concentrations of Brassinolide were distributed in the  sub-plots. A significant effect of the cultivars and the concentrations of the Brassinolide on the studied response variables. The sprayed plants with 1.5 mg L^-1 concentration achieved highest number of seeds per disc (833 seed disc^-1), weight of 1000 seeds (65.08 g), percentage of fertility in the seeds (93.89%), total seed yield (3573 kg ha^-1) and oil yield (1586 kg ha^-1). The vareities were significantly different for all studied traits. Sakha cultivar recorded the highest values ​​for the number of seeds per disc, the percentage of fertility in the seeds, the total yield of seeds and the oil yield (708 seed disc^-1, 93.05%, 2810 kg ha^-1, 1217 kg ha^-1, respectively), whereas Ishaqi 1 was superior in terms of the weight of 1000 seeds (61.33 g) and Akmaar was superior in terms of oil content (46.16%). Sakha cultivar was the most responsive to the addition of Brassinolide and achieved a high average for the seed yield and oil.