The Paulownia tomentosa tree is one of the quickest developing and most adaptative hardwood trees on the planet. Given the horticultural and business significance of the paulownia trees, this study was carried out in Al-Zahriya Garden at the Horticultural Institute of Agricultural Research Center in Giza, Egypt between 2018 to2020. This study was conducted in two phases, the first phase investigates the different effects of different levels of Auxin on the rooting process and the second phase explores different soil mixtures' effects on the acclimatization of the plant. Vegetative plants were obtained from pre-cultured tissues from the same laboratory and the same flower garden. In phase1, three different types of Auxins were used – IBA, NAA and IAA with concentration levels of 0mg/L, as a control phase, 1mg/L, 2mg/L and 3 mg/L. At control concentration [0mg/L], The experiment was conducted by this study to find out the best protocol for in vitro and in vevo micropropagation of Paulownia Tomentosa. The results showed that the IBA at 2 mg/L had the highest position for shoot fresh weight. NAA at 2 mg/L: acquired the highest number for shoot length, shoot fresh weight, root fresh weight and total chlorophyll content. IAA at 1 mg/L: got the most elevated grade for total chlorophyll content. IAA at 2 mg/L: had the most elevated degree for shoot length. IAA at 3 mg/L: attained the most elevated grade for the number of shoots and leaves. Potting Mix 3(Peat moss + Perlite + Vermiculite): Highest for Fresh Castor Weight.