A relibale protocol for in vitro propagation of husk tomato (Physalis txocetp«,
L.) planl var. Americana was established. Shoot tips explants were used and different
cytokinins were compared. Shoot prolifer~!:'Jn was obtained from shoot tips at
relc:tively height frequency when cultured on Murashige & Skoog (MS) medium
supplemented with high level of cytokinins (4.0 mgfl). while it had a great inhibitory
effect on root initiation. Benzytadenine (SA) surpassed significantly than Kinitin (Kn) in
stimulating of the lateral bud- break and increasing shoot branching in the plants.
Seedlings derived from tissue culture produced plantiets which were shorter. thicker.
with more leaf chlorophyll content, less leaf temperature, transpiration, bigger leaf
area and gave higher total yield than Ihose derived from seed sowing