Shoot tips of Gerbera jamesonii H. Bolus were used as explants in this study. Aseptic cultures of Gerbera were established by placing the isolated shoot tips on
.Murashige and skoog medium supplemented with different cytokinins (SA, Kin or 2ip)
at different concentrations. The highest number of shoots/explant and the heaviest c fresh weight/culture were obtained when MS medium contained 2.0 mg/L SA. Leaves
of the obtained aseptic cultures were examined as explants to produce shoots (direct
organogenesis). Interestingly, while intact leaves showed positive response in shoot
formations on the petioles, there were no shoot formations on cut leaves (without
petioles). This study recommended using intact old leaves of gerbera as they showed
higher response on shoot formations when compared with the response of young
leaves. While, the highest number of shoots was obtained on the petiole when the
intact old leaf was cultured on MS medium contained 3 mg/L BA, the highest
percentage of old leaves, that produced shoots, was achieved with the medium
-contained 1, 2 or 3 mg/L SA. While at rooting stage, the highest response of root
formations was achieved when the medium supplemented with 0.5 mg/L ISA, in the
same time proved to be the most effective in increasing the root lengths. The plantlets
were successfully acclimatized when transferred to pots contained soil mixer of
'peatrnoss: sand : vermiculite in equal volumes (1: 1: 1). The survival percentage was
100% after one month from tans planting. Abbreviations: MS: Murashige and Skoog; 2,4-D : dichlorophenoxyacetic acid; SA: Senzyladenine; NAA: naphthalene acetic acid; IBA: indole-3-butyric
acid; Kin: Kinetin; 2ip (2-isopenteyladenine .