Infectivity test and indirect ELISA were used for detection of alfalfa mosaic alfamovirus (AMY) in flowers, pods, intact seeds and seed parts of alfalfa cv. EI-Wadi EI-Gadid during maturation. Infective virus incidence determined by infectivity test or vital antigen Incidence determined by indirect ELISA decreased with maturation. ELISA was more sensitive than infectivity test for detection AMV in flowers, pods, seeds, seed coats and embryos. Higher percentage of AMV infection was detected in male sex organ (androecium) as compared with the female one (gynoecium) .Infectivity test did not detect infective virus in the separated seed coats. Indirect ELISA showed that higher proportion of embryos contained AMV antigen as compared with seed coats.Results showed that alfalfa seeds contain virus inhibitors. Seed extracts diluted at 1 :20 (w/v) significantly reduced number of local lesions produced by AMV, whereas t~ Jse diluted at 1: 1 00 and 1 :250 did not affect lesion counts.