The experiments have been achieved to examine a method to obtain
homogenization using gametophytes as well as studying the effect of different culture
media composition on sporophyte formation of Nep < /em>hro/ep < /em>is exaltata. The statistical
analysis of data revealed the following findings: Spores were germinated after two
weeks at 1/2 strength MS medium supplemented with agar at 1.5 gIL. In the first
experiment (multiplication of the gametophytes colonies), the highest significant
values of fresh weight (16.19 g) and length (6.02 ern) were obtained after five months
as a result of modifying 3/4 strength MS medium with 2 mglL kinetin + 0.1 mglL NAA.
In the second experiment in these stages. the highest significant fresh weight
of gametophytes colonies value (17.66 g) was obtained as a-result of modifying MS
medium with 4 mglL SAP + 0.2 mglL IAA after three months from spores germination.
In sporophytes formation stage, MS 1/2 strength medium supplemented with 3
gmlsucrose + 3 giL AC induced the highest significant gametophyte colonies diameter
(5 cm) in most treatments. However, MS 1/4 strength supplemented with 15 g sucrose
recorded the lowest diameter value to about 2.66 cm. As for the percentage of
gametophytes colonies showing sporophytes, the combination of MS 1/2 strength
medium supplemented with sucrose (15 giL) significantly increased percentage of
gametophytes colonies showing sporophytes formation of about 88%, while
gametophytes which were cultured on MS 1/4 strength medium supplemented with
sucrose (30 giL) nearly failed induce sporophytes. In hardening off stage, the highest
significant survival percentage of hardened young sporophytes (99.91) was obtained
in case of using 2 peat + 2 loam + 1 sand by volume culture medium. The best
vegetative growth was reported with 2 peat + 2 loam + 2 sand medium (24 6 cm
height, 47.66 fronds and 6.20 g dry weight) per plant. Also the same medium
significantly increased values of chlorophyll, total carbohydrates, and total indoles.
It could be concluded through this research, a fast life cycle and high
sporophyte production on Nephrolep < /em>is exaltata, homogenization of gametophytes can
be considered to be an excellent method for propagation, yielding incomparable
numbers of sporophytes relatively in short period of time.