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237932

INTENSIVE PRODUCTION OF DIHAPLOID PLANTS FROM Cucurbita pepo L. THROUGH ANTHER CULTURE TECHNIQUE.

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Last updated: 22 Jan 2023

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Abstract

Production of haploid plants using techniques such as anthers culture has
long been considered important fOf gene~ic studies and plant breeders. Since haploid
plants carry only one allele of each gene, recessive characteristics are apparent.
These haploid plants can be used to produce homozygous dihaploid plants useful for
plant breeding. This studv is concerned with the production of dihaploids plants of
Cucurbita p < /em>epo L. Anthers at mid to late uninucleate microspore stage without
filament were excised from sterilized buds of three genotypes of summer squash: i. e
Gabbla, Eskandarani and Rosina F,. Anthers were cultured on two different induction
media. It was found that both media and cultivars affected callus induction and
plantlets regeneration. The cullivar Gabbla gave the maximum callus weight (0.450 g)
on MS medium supplemented with 5.0 mg II 2.4-0 and 90 gIl sucrose. On the other
hand, the cultivar Eskandarani gave the maximum number of plantlets per dish
(21.3)and per callus (4.3). Plantlets were acclimatized in the controlled environment.
In each genotype, the root tips of plantlets were examined for ploidy level. Results
reveal that there was 50% haploid plants in Eskandarani and Gabbla cultivars while
Rosina F 1 had 40% haploid plants.

DOI

10.21608/jpp.2005.237932

Authors

First Name

M.

Last Name

Badawi,

MiddleName

A.

Affiliation

• Department of Vegetable Crops, Faculty of Agric., Cairo University, Egypt.

Email

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City

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Orcid

-

First Name

E.

Last Name

Metwally

MiddleName

I.

Affiliation

Horticulture Department, Faculty of Agriculture, Tanta University, Kafr EI-Sheikh, Egypt

Email

-

City

-

Orcid

-

First Name

S.

Last Name

Taha

MiddleName

S.

Affiliation

• Department of Vegetable Crops, Faculty of Agric., Cairo University, Egypt.

Email

-

City

-

Orcid

-

First Name

M.

Last Name

Arafeh

MiddleName

O.

Affiliation

• Department of Vegetable Crops, Faculty of Agric., Cairo University, Egypt.

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City

-

Orcid

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Volume

30

Article Issue

12

Related Issue

34280

Issue Date

2005-12-01

Receive Date

2005-11-15

Publish Date

2005-12-01

Page Start

7,885

Page End

7,893

Print ISSN

2090-3669

Online ISSN

2090-374X

Article File

JPP_Volume 30_Issue 12_Pages 7885-7893.pdf

PDF . 143.9kB

Link

https://jpp.journals.ekb.eg/article_237932.html

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https://jpp.journals.ekb.eg/service?article_code=237932

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5

Type

Original Article

Type Code

887

Publication Type

Journal

Publication Title

Journal of Plant Production

Publication Link

https://jpp.journals.ekb.eg/

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Article

Created At

22 Jan 2023