This study was conducted in two winter seasons of 2002-2003 and 2003-2004 on cucumber hybrid "PRINCE" at EL-Borolos area under clear polyethylene low tunnel conditions to evaluate the effect of some organic manures (Chicken manure and compost) and biofertilizers (dual inoculation of Azotobacter and Azospirillum) compared with chemical fertilizers on growth, early and total yield, fruit characteristic and chemical composition (NPK), cholorophyll and the rhizospheric microbial symptoms. (Nitrogenase activity, count of associative diazotrophs, Azotobacter spp and Azospirillum spp, CO₂ evaluation, soil dehydrogenase activity and Bacterial population. Results indicated that application of chicken manure with biofertilizer at the rates of 10 m³ and 20 m³/ feddan significantly increased vegetative growth (plant height, number of branches, number of leaves and leaf area), early and total yield and fruit quality. Furthermore, rhizosphere microbial symptoms (nitrogenase activity, number of associative diazotrophs, Azotobacter, Azospirillum spp., CO₂ evolution, dehydrogenase activity and bacterial population) were also increased compared with those resulted in chemical fertilizer application. As for the chemical contents of cucumber leaves and fruits (N, P and K and total chlorophyll), results showed that N, P and K were similar in all treatments. Chlorophyll content did not differ significantly from control.