When using cheaper substrates instead of agar, perlite gave the significant heaviest Gerbera shoots, the highest number of shoots and the greatest significant leaf number. Gerbera shoots grown on perlite had the highest content of total chlorophyll.
In case of Lilium, the significant heaviest shoots and the highest number of shoots were a result of using cotton as a substrate. The effect of substrate type on shoot length was significant on Lilium. Longest Lilium shoots were those grown on peat moss. Greatest significant leaf number and the highest content of total chlorophyll were detected in shoots grown on cotton.