This study aimed at investigating the effect of ammonium sulfate (20.6% N) at 300 Kg/fed, calcium superphosphate (16.5% P205) at 200 Kg/fed, plant nutrients (Nofatrein) as foliar spray and bio-fertilization (Microbein) on growth and chemical composition of Euryops pectinatus (L.) Cass. The treatments were 100% NP (AS CONTROL), 75% NP, Microbein alone, Microbein+ 75% or 50% of soil application, Nofatrein alone, Nofatrein + 75% or 50% of soil application, Microbein+ Nofatrein and Microbein+Nofatrein+50°/o of soil application. All the treatments received constant dose of potassium sulfate (48% K20) at 150 Kg/fed (recommended) but the plants received different percentages of the full NP rate (NP 100%) . The field experiments were carried Out during the two successive seasons of 2004/2005 and 2005/2006 at the Experimental Nursery of the Ornamental Horticulture Department, Faculty of Agriculture, Cairo University. There was a significant increase in plant height by using the combination of bio-fertilizer and 75% of the chemical soil application. Also, combining Microbein with 50% of the soil chemical fertilizers and Nofatrein significantly increased plant height and stem diameter. Applying the foliar fertilizer Nofatrein caused the maximum number of branches per plant and stem diameter in both seasons. There were three treatments (viz. Nofatrein alone. the treatment of 75% chemical fertilization combined with Microbein, and the combination of 50% chemical fertilization + Microbein + Nofatrein) that gave the best result in terms of plant fresh and dry weights, with insignificant differences between them. The highest number of flowers per plant, root fresh and dry weights and total chlorophyll content were obtained from plants supplied with 50% chemical soil fertilization, combined with Microbein and Nofatrein.