A field experiment was conducted in South Tahrir, Eiehira Governorate, Egypt, to investigate the effect of different types of organic fertilization on ten quantitative characters among 15 basil genotypes belonging to three species of basil. Treatment types of organic fertilization were (0), (T1), (T2) and (T3). The genetic variation was observed to be high among genotypes and fertilizers for all studied characters in both generations. G.C.V% values were high for LG, LFW, HFW, HDW, E096 and EOY in two generations. Very high heritability values were recorded for SDW and EOYlpIant. However, moderate heritability values were noted for SFW and HFW in both generations. As similar, the genetic advance was observed to be high for, LG, LFW and HFW; these results indicated the gene expression response for organic fertilization in selected genotypes of basil. Association analysis of studied characters revealed high significant positive correlation between essential oil yield with NPB, LFW, LDW, HOW, and EO%,. Regarding to the mean perforr'ha nce of different studied traits, superior genotypes were identified for direct or further use in breeding programs to improve basil species under organic fertilization.