236111

ANTHER CULTURE AS A TECHNIQUE TO REGENERATE HOMOZYGOUS WHEAT (Triticum aestivum L.) GENOTYPES

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Last updated: 04 Jan 2025

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Abstract

Eleven wheat (Triticurn aestivum L.) genotypes were screened for salinity tolerance. Seeds were germinated under six salinity levels (0 — 12000 ppm) with quarter strength of Hogland solution. Sea salt was used as a source for salinity. Three genotypes, i.e. Sids 1, Sakha 8 and line 25 were selected for performing crosses among the salinity tolerant (Sids 1 and Sakha B) and the salinity sensitive Line 25. Three crosses were conducted between the selected genotypes and their F1 plants were grown to obtain their anthers. Anther culture was used as a breeding technique to obtain pure lines in a short time. P-48 medium was used for callus induction. Great differences were observed in callus induction among the anthers of the three crosses. Embryogenic callus induction reached 10.1, 61.9 and 51.4 % for hybrid 1 (Sids 1 x Sakha 8), hybrid 2 (Sakha 8 X Line 25), and hybrid 3 ( Sakha 8 X Line 25), reSpectively. Plant regeneration medium 190-2 was used. Frequency of regenerated plants reached 1.4, 8.5, and 9.0 % for crosses 1, 2, and 3, reSpectively. Root tips of regenerated plants were cytologically examined to determine their ploidy level. All the examined plants were haploid type. Haploid plants were treated by colchicine for chromosome doubling. The doubled haploid plants reached 22.3, 24.4, and 22.1 % for crosses 1, 2 and 3 respectively.

DOI

10.21608/jpp.2006.236111

Authors

First Name

S.

Last Name

Sabry,

MiddleName

R.S.

Affiliation

Wheat Research Department, Field Crops Research Institute, Agricultural Research Center (ARC), Giza.

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First Name

O.

Last Name

EI-Shihy

MiddleName

M.

Affiliation

Plant Physiology Section, Agricultural Botany Department, Faculty of Agriculture. Cairo Unvi., Giza.

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City

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Orcid

-

First Name

M.

Last Name

Nesiem

MiddleName

R.A.

Affiliation

Plant Physiology Section, Agricultural Botany Department, Faculty of Agriculture. Cairo Unvi., Giza.

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City

-

Orcid

-

First Name

D.

Last Name

Daghma

MiddleName

S.

Affiliation

National Gene Bank and Genetic Resources (NGBGR), Ministry of Agriculture and Land Reclamation, Giza.

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Volume

31

Article Issue

8

Related Issue

33986

Issue Date

2006-08-01

Receive Date

2006-07-19

Publish Date

2006-08-01

Page Start

5,081

Page End

5,092

Print ISSN

2090-3669

Online ISSN

2090-374X

Link

https://jpp.journals.ekb.eg/article_236111.html

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https://jpp.journals.ekb.eg/service?article_code=236111

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17

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Original Article

Type Code

887

Publication Type

Journal

Publication Title

Journal of Plant Production

Publication Link

https://jpp.journals.ekb.eg/

MainTitle

ANTHER CULTURE AS A TECHNIQUE TO REGENERATE HOMOZYGOUS WHEAT (Triticum aestivum L.) GENOTYPES

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Article

Created At

22 Jan 2023