Two field experiments were carried out at Gemmeiza Agricultural Research
station, EI-Gharbia Governorate during two seasons (2002 and 2005). The main
objective of this investigation was to study the effect of bio-fertilizer (Rhizobacterein)
as a method to decrease the levels of mineral nitrogen fertilizer of Giza 89 cotton
variety using 60 kg/fed. without Rhizobacterein (as control) and using 30, and 45
N/fed. with Rhizobacterein at thinning, squaring and flowering stages On some
cotton plant growth traits and attributes, earliness seed cotton yield / fed. and its
components and net income. Results can be summarized as follows: Application of bio-fertilizer (Rhizobacterein)1 at treatment (8) [45 kg N/fed. +
Rhizo. (2 L.Ifed. squaring) + 2 L.Ifed. flowering stage]. Significantly increased plant
height, number of fruiting branches / plant, boll weight, number of open bolls / plant,
seed cotton yield / fed. and lint percentage in both seasons. Also, applying bio-
fertilization significantly increased the net income / fed. as compared with the check
treatment. Also, using bio-fertilizers Significantly increased dry weight of plant organs
during different growth stages and significantly increased leave contents of
chlorophyll, carotinoids, nitrogen, oil and protein contents in seeds.