Alternan‘a sp., Aspergilius niger, Peniciliium sp. and Pseudomonas sp. were isolated from Phoenix dactylifera cv. Zaglol culturing in vitro in starting. maturation 3. germination and multiplication stages. Cupper oxychlonde, Vitavax T, Topsin M, Streptomycin. Banucin and Bencid D. were used to reduce the contamination with fungi and bacteria. Using streptomycin at the concentration 250 ppm stages was more effective in increased survival percentage and decreased the degree of contaminated explants by bacteria on starting, maturation a. germination and multiplication. On the other hand, using Cupper oxychlon'de, vitavax and topsin at 500 ppm for each on starting stage, maturation 8. germination and multiplication stages recorded no contamination by fungi. Topsin at 250 ppm gave the best results treatments comparing the other treatments results on the explants starting stage it obtained the highest degree of swelling and the lowest degree of browning. Where as. on maturation 3. germination and multiplication stages treated by using Streptomysin at 50ppm which gave the highest degree of growth vigor and the lowest degree of browning.