This stydy was carried out to study the effect of NPK fertilization and application of some soil amendment agents on vegetative growth and yield of Thompson Seedless grapevines in three years 2002, 2003 and 2004. Results were taken in the second and third season. Thirteen treatments were applied. Three doses of N with one dose of K, P, gypsum or sulphur. Each N dose was applied in three times as equal ratios, at the first of February and mid April and May, respectively. While. P fertilizer was applied with the application of gypsum or sulphur in November. K fertilizer was applied once at the first application of nitrogen on February. Soil application with gypsum or sulphur led tol‘irgprove most soil physical and chemical properties. Adding gypsum or sulphur to the fertilization program gave the highest shoot length and best root distribution as number of fibrous roots and fresh weight (g) in the upper layer (0-30) cm and at (30-50) cm depth compared to the control in three seasons. Higher nitrogen rate (N3) with gypsum or sulphur treatments led to improve nutrient status as leaf chlorophyll a and b values total carbohydrate and leaf petioles mineral contents especially N, P, Ca and Mg in the three seasons of study. Moderate nitrogen rate (N2) with gypsum or sulphur treatments produced higher yield as number of clusters and weight {g)ivine and improved most physical and chemical properties of clusters and berries juice TSS value. Thus, the best treatment in this study was by adding Moderate nitrogen rate (N2) with gypsum or sulphur treatments to the fertilization program to gain maximum yield with good cluster quality for Thompson seedless grapevines.