Fusarium oxysporum, Phomopsis sp., Fusan'um monififorme and Fusarium soiani were isolated from leaves, basal of leaves, crown area and roots of date palm tissue culture (Phoenix dactylifera L.) and found to be pathogenic to it. The   plants showing wilt of leaves, necrosis at the basal of leaves, canker of crown area and heart rot symptoms in greenhouse (2 years after acclimatization). Fixed section through infected tissues showed that the fungi caused necrotic area and gum block in vascular bundle and paranchymatous (F. oxysporum). Tylosis like bodies in metaxylem vessel (Phomopsis sp.) while the mycelium of F. moniiifonne and F. soiani were found inter- and intracellular paranchymatous tissues. All tested fungi destroyed some of vascular bundles and paranchymatic cells which were filled with brown materials. Free and conjugated phenol contents were higher in infected tissues than determined in health ones, while the sugar contents were reduced in infected fissues.