Five Egyptian cotton varieties Giza 70,Giza 85, Giza 88, Giza89 and Giza 90
were crossed in complete diallele crosses meeting design and produced 20 F1s
genotypes. The genetic variability and relationships among 11 cotton genotypes (five
cotton varieties and six F_1s) were achieved using SOS-PAGE and 10 RAPO primers.
The total number of bands which produced by SOS-PAGE ranged from 17 with Giza
85 to 23 with G3 (Giza70), and the cluster analysis divided the eleven cotton
genotypes into two groups. The level of polymorphism for all genotypes, as revealed
by RAPO was 77.05%. In addition, RAPO analysis revealed different genetic similarity
( according to Dice coefficient). Among the 11 cotton genotypes the highest similarity
was between G7 and G8. However, the G8 was 61.3% similar to its female parent
G90 and 78% to its male parent G89, while the lowest genetic similarity was between
G6 and G10 (54.5%). Three of five Egyptian cotton varieties were characterized with
eight positive and one negative unique RAPO markers.