The best sterilizing treatment for Dieffenbachia picta cv Tropica buds was immersion for 30 min. in fungicide solution of Rizolex (2 g / L), followed by soaking in  0.3 % mercuric chloride solution for 20 min., then transferred to sodium hypochlorite solution at 1.0 % for 30 min. Establishment stage period could be decreased from 8 weeks to be only 4 weeks by culturing the buds on MS medium supplemented with (16 2ip  + 2 IAA + 1 DPU mg/L) which also increased the number of shoot /explant. Different concentrations of triiodobenzoic acid (0.0, 1.0, 2.0, 4.0 or 8.0 mg/L), malt extract (0.0, 1.0, 2.0, 4.0 or 8.0 g\L), yeast extract (0.0, 1.0, 2.0, 4.0 or 8.0 g\L) or casein hydrolysate (0.0, 0.50, 1.0, 2.0 or 4.0 g\L) combined with (16 2ip + 2 IAA + 1 DPU mg/L) were tested during multiplication stage. The best treatment for shoot multiplication (10 shoots /explant) and rooting (4 roots /shoot with 6.48 cm root length) was 4 g/l malt extract combined with (16 2ip + 2 l IAA + 1 DPU mg/L). Plantlets were successfully acclimatized (95% survival percentage) in peat moss and sand (1:1, v/v).