A field experiment was conducted at the farm of Faculty of Agriculture, Moshtohor, Benha University during the 1998/1999 and 1999/2000 winter seasons. The study aimed to evaluate the productive characteristics of two squash cultivars (Eskandarani and Arlika F1 hybrid) that fertilized through 100% biogas fertilizer, 50% biogas + 50% mineral fertilizer and 100% mineral fertilizer on nitrogen basis (60 kg N/ feddan) with or without seed vernalization. Treatments were arranged in split plot design w