The present work was carried at Baramoon Experimental Farm, Dakhalia Governorate, Egypt, during the two spring seasons of 2004 and 2005. Potatoes cv. Spunta was cultivated to study theeffect of glycine, lysine and nitrogen fertilizer rates on growth, yield and chemical composition of potato , i.e., (control, glycine at dose 100 ppm, lysine at dose 100 ppm, a mixture of glycine 100 ppm plus lysine 100 ppm) and nitrogen rates of 120, 150 and 180 kg/fed. The results showed that nitrogen fertilizers rates increased significantly vegetative growth parameters, i.e., plant height, number of main stems, foliage fresh and dry weight at 75 days after planting, in addition to total tuber yield, tuber number, tuber weight/plant, NPK in leaves at 75 days after planting and P, dry matter, starch, protein contents in the tubers in two seasons. The best growth parameters of potatoes were recorded with foliar addition of glycine  plus lysine each at 100 ppm. The highest total tuber yield, tuber number, tuber weight, NPK in the leaves at 75 days after planting and tuber at harvest time, dry matter, starch as well as protein content in the tuber were obtained by glycine plus lysine treatment in both seasons. The interaction between N fertilizer rates and amino acid,  had significant effect on all vegetative growth parameters, total tuber yield and its components, NPK in the leaves and tubers, dry matter, starch and protein contents in the tubers except N in the first season.