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220905

GENETIC IMPROVEMENT OF HIGHLY GELLAN GUM PRODUCING STRAINS OF Sphingomonas paucimobilis via PROTOPLAST FUSION

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Last updated: 04 Jan 2025

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Abstract

Three local strains; Sphingomonas paucimobilis Kb1( S.p.Kb1), Sphingomonas paucimobilis CAAS11, (S.p.CAAS11) and Sphingomonas paucimobilis .CAAS6 S.p (CAAS6) were used throughout this investigation to improve their potentialities as gellan gum producers. Three of protoplast fusion experiments were done between the three Sphingomonas paucimobilis local strains; S.p.Kb1 (P1), CAAS6 (P2) and S.p.CAAS11 (P3) at all possible combinations and the fusants were obtained after 1 hr incubation period. Four fusants were obtained after one hour producing gellan gum higher than their parents. The obtained fusant Sp2::Sp3- F4 from the third protoplast fusion experiment P2:: P3  is the best in their gum efficiency rate (two times and half than their parents), followed by the two obtained fusants; Sp1::Sp2- F1 and Sp1::Sp3- F2, from the other two experiments; P1 :: P2 and P1 :: P3, respectively have the same gum efficiency rate, two times than their parents. They have higher viscosity values than that of their parents. All the obtained four fusants have promising economic value and can be used from further genetic improvement.

DOI

10.21608/jpp.2007.220905

Keywords

Sphingomonas paucimobilis, protoplast fusion, Polysaccharides, Gellan gum, Viscosity and genetic improvement

Authors

First Name

S.

Last Name

Abd-El-Aal,

MiddleName

kh.

Affiliation

Microbial Genetic Dept., National Research Center, Cairo, EGYPT

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First Name

M.

Last Name

Asker

MiddleName

S.

Affiliation

Microbial Biotechnology Dept., National Research Center, Cairo, Egypt

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Volume

32

Article Issue

10

Related Issue

31484

Issue Date

2007-10-01

Receive Date

2007-09-14

Publish Date

2007-10-01

Page Start

8,263

Page End

8,271

Print ISSN

2090-3669

Online ISSN

2090-374X

Link

https://jpp.journals.ekb.eg/article_220905.html

Detail API

https://jpp.journals.ekb.eg/service?article_code=220905

Order

14

Type

Original Article

Type Code

887

Publication Type

Journal

Publication Title

Journal of Plant Production

Publication Link

https://jpp.journals.ekb.eg/

MainTitle

GENETIC IMPROVEMENT OF HIGHLY GELLAN GUM PRODUCING STRAINS OF Sphingomonas paucimobilis via PROTOPLAST FUSION

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Article

Created At

22 Jan 2023