This present investigation was carried out to monitor and follow the protein content and concentration tolerant plants against salinity and drought in comparing to explant donor (GT54-9) and its non tolerant clones. SDS-PAGE technique and proline determination were performed to study the differences among sugarcane variety GT54-9, and its somaclones which produced through tissue culture technique and were tolerant to drought and salinity. The obtained results could be summarized that differentiation in proline concentrations were observed for GT54-9, control, somaclones of GT54-9, GT54-9 salinity and drought tolerant mutants which were 17, 37, 55 and 115 mmol respect. Protein band with 67 KDa which presence in all samples was absence in salinity tolerant plant. Also, protein bands with 50 KDa which expressed in control plant and drought tolerant plant were none expressed in salinity tolerant plant and somaclones. Furthermore, protein band with 32 and 21 KDa was recorded in drought tolerant plant and somaclones plant. Then, disappeared in control and salinity tolerant plant. In final, 20 KDa protein band was only presence in control plant.  Base on Total lab program, many of protein bands which have the same molecular weight were varied in protein concentration for different plants under study.