This study was carried out to disclose the possibility of replacing mineral fertilizer requirements of Flame seedless grapevines by organic fertilizer (chicken manure) and to study its effect on yield, bunch characteristics, shelf life and residues of NO₃^- and NO₂ in the juice of berries. Different levels of nitrogen 30, 40 and 50 nitrogen units/feddan were used. The experimental vines received different combinations of both mineral and organic N fertilizers as follows : 1-    100 % mineral N fertilizer. 2-    75 % mineral N fertilizer + 25 % organic N fertilizer. 3-    50 % mineral N fertilizer + 50 % organic N fertilizer. 4-    25 % mineral N fertilizer + 75 % organic N fertilizer. This study revealed that replacing 75 % N mineral fertilizer requirements by organic fertilizer at 50 N units significantly increased leaf area, total chlorophyll, leaf nitrogen, potassium and phosphorus content. Also, this treatment increased the yield per vine, cluster weight and size, berry weight and size and juice volume, SSC, SSC/acid ratio and total anthocyanin in berry skin and decreased acidity. On the contrary, it decreased nitrate and nitrite in berry juice than mineral nitrogen fertilizer alone. From this study, it is clear that using organic fertilizer as a partial substitute for mineral nitrogen of Flame seedless grapevine at 50 unit N/feddan had a positive effect on all characters measured. So, it can be recommend to use the treatment of 75 % organic fertilizer + 25 % mineral N fertilizer at the level of 50 N units/fed. as the best treatment under the experiment conditions. Since, it gave the highest yield with better cluster and berry characteristics.