Two field experiments were carried out during the two successive seasons of 2005 and 2006 at Banha ( Qalubia Governorate ) to study the effect of rates of poultry manure and chemical nitrogen fertilizer with or without Biofertilizer " Nitrobin " at rates of 500 g./fed. on growth, yield, quality and chemical contents of tomato plants cv. Gs 12. Obtained data showed that using 50 % poultry manure + 50 % mineral fertilizer or 25 % poultry manure + 75 % mineral fertilizer increasing significantly all vegetative growth characteristics (plant length, number of leaves / plant and number of shoots / plants) in the two seasons of study. The highest yield and fruit quality (fruit length, fruit diameter, fruit weight and T.S.S) was found by 25 % poultry manure + 75 % nitrogen mineral fertilizer or 50 % poultry manure + 50 % mineral fertilizer. Using biofertilizer (Nitrobin) increased significantly the vegetative growth characters (plant length, number of leaves and shoots/ plant). Also,using biofertilizer increased significantly the total yield and quality of tomato fruits.