The present investigation was carried out in the Experimental Station and Laboratory of the Vegetable Crops and Ornamental Plants Department, Faculty of Agriculture, Mansoura Univ., Egypt, during the two successive seasons of 2002/03 and 2003/04 to study the effect of farmyard manure    (10, 20 and 30 m³ /fed.) and bio-fertilizers (Microbein, Phosphorein and Rhizobacterein) on Plantago ovata Forsk plants. The results indicated that FYM doses had a positive effect on plant height, number of tillers and spikes, dry weight per plant, seed yield per plant and plot as well as mucilage content and percentage when compared with each other. The best results were of plants which received the medium FYM dose (20 m³ /fed). The inoculating with Rhizobacterein bio-fertilizer gave the tallest plants, while the inoculating with Microbein gave the highest number of tillers and spikes, dry weight per plant, seed yield per plant and plot as well as mucilage content and percentage. The combined treatment of the medium FYM dose and Rhizobacterein inoculating gave the tallest plants, while the combined treatment of the medium FYM dose and Microbein gave the highest number of tillers and spikes, dry weight per plant, seed yield per plant and plot as well as mucilage content and percentage. Also, the combined treatment of the medium FYM dose and Phosphorein gave the heaviest 1000 seed weight.