A field trail was carried out in sandy soil at Borollous location North Nile Delta, Kafr EL-Sheikh Governorate during two successive summer seasons of 2003 and 2004. The study concerned with the effect of different types and levels of organic manures combined with inoculation of tomato plants with Mycorrhiza and Azospirillum as biofertilizers on plant growth, yield and its components as well as fruit quality. Besides, the microbiological activity in rhizosphere area of tomato root in terms of Mycorrhiza root colonization, the cell counts of Azospirillum and nitrogenous activity were also studied. Results revealed that the highest vegetative growth i.e. stem length, number of branches and leaves as well as fresh and dry weight of tomato plant were obtained by applying 10 ton/fed. of chicken manure + biofertilizers. On the contrary the lowest values were recorded with the other compost treatments included in this study i.e. Obour, Biogreen and EL-Arish with biofertilizer.              Regarding plant chemical constituents, i.e. NPK, chlorophyll as well as fruit chemical contents, i.e. acidity, Vitamin C and TSS, results showed that the leaf content of chlorophyll as well as N, P, were higher by using chicken manure combined with bio fertilizer than the other studied types of compost. The concentration of vitamin C in tomato fruit increased significantly by using chicken manure combined with Bio-fertilizers. Tomato plants fertilized with chicken manure at a rate of 8 ton /fed. combined with biofertilizer produced the highest fruit yield as compared with the other tested treatments in this study. Concerning root rhizosphere tomato microbial activity, results revealed that all tested treatments increased significantly all microbial tested parameters over the control in both seasons. However, using chicken manure + biofertilizers gave the highest percentage of root Mycorrhiza colonization, as well as cell counts of Azospirillum and nitrogenase activity compared to the other tested organic manures + biofertilizer including control treatment. It can be recommended that chicken manure at a rate of 8 ton /fed + biofertilizer, which gave the highest fruit yield with best quality as well as microbial activity for the root rhizosphere, can be used for tomato cultivation in sandy soil. Moreover, producing tomato crop free from any chemical residues, through avoiding the use of chemical fertilizers.