This study aimed to investigate the effects of both bio-and mineral fertilizers on certain morphological, physiological and anatomical aspects as well as yield and its components of potato plant. The most important results achieved are summarized as follows: All growth parameters expressed by plant height, number of branches and leaves per plant, leaf area of potato plants, photosynthetic pigments, carbohydrate fraction the shoot system  as well as yield and its components were deceased with decreasing the level of NPK less than the recommended dose (control) All bacterial strains increased most of the plant growth parameters. Inoculation with NFB strain individually or in combination with other strain was most effective in this respect. Moreover, all bacterial strains used showed an additive effects to the effect of 100% NPK on potatoes growth. Anatomically, inoculation of bacterial strains used, over all NPK doses increased leaflet thickness in the midrib region, mesophyll tissue thickness, midrib V.B. dimension, xylem, phloem tissue thickness and metaxylem vessel dimension. Moreover, stem diameter, cortex thickness, large vascular bundle dimensions, external and internal pholem and xylem tissues thickness as well as pith tissue dimension were also increased. The results indicate that, all the anatomical parameters studied of the leaf and stem were decreased compared with the control (100% from the recommended dose). The decrease was a concentration dependent.