This work deals with the effect of Fusarium culmorum toxin on the induction of callus, regeneration and root induction during tissue culture of mature embryos of four Egyptian wheat (Triticum aestivum) genotypes, i.e. Sids 1, Sids 8, Sids 9 and Giza 167. The two concentrations of F. culmorum toxic filtrate (10% and 20%) in addition to the control were applied to treat mature embryos and their calli. All genotypes; turned to be very sensitive to the toxic filtrate, but it was found that the level of sensitivity depends on genotype. The four genotypes differed from one another significantly in their ability to induce root formation, the highest response was observed in Giza 167. But, there were no significant differences due to the toxic medium and toxic medium X genotype interaction. It was concluded that the highest ability of root induction (in the presence of toxic filtrate) and the highest percentage of survival calli were encountered for Giza 167, and the lowest values were obtained for Sids 1. Whereas, the percentage of callus induction (in the presence of toxic filtrate) was determined in the highest values for Sids1.