Peach rosette mosaic virus (PRMV) was detected in naturally infested grapevine (Vitis vinifera cv. Superior) plants growing in the Horticulture Institute Experimental Station Giza Governorate. The virus was found, then to be transmitted by mechanical, nematode (Xiphinema americanum and Meloidogyne incognita) and graft inoculation. Double antibody sandwich enzyme linked immunosorbent assay (DAS- ELISA) was used to detect the presence of virus and to confirm the transmission. As for X. americanum, its effect was more pronounced at the highest nematode inoculum level than in plants infected with virus added to the lowest nematode level.  Moreover, on grape plants infected with M. incognita, nematode inoculum levels significantly (P≤ o.o5 and 0.01) increased when nematodes were inoculated alone than when nematode and virus were inoculated simultaneously (where virus source and virus-free bait plants were grown simultaneously in the same pot).