Two field experiments were carried out at Sakha Agricultural Research Station, during 2001/02 and 2002/03 seasons to investigate the response of four barley cultivars (Giza 123, Giza 126, Giza 2000 and line 2) to moisture stress at different growth stages (one irrigation was applied at sowing; two irrigations were applied, the first at sowing and the second after 45 days from sowing and three irrigations were applied at sowing, after 45 days and 75 days from sowing) and three nitrogen levels (0, 18 and 35 kg N/fed.). The experimental design was a split-split plot, where the irrigation treatments were allocated to the main plots and nitrogen levels arranged to the sub plots, while the four cultivars occupied the sub-sub plots.                 The results showed that increasing number of irrigation significantly increased grain yield and its components and also the traits under this investigation. Increasing nitrogen level from 0 to 35 kg N/fed. led to significant increases in all studied traits except in case of grain ash percentage, grain moisture content and relative density of grain barley. The results also showed that Giza 123 had the highest values of grain yield, yield components and all grain quality traits under this studies. It could be concluded that cultivation of Giza 123 under 35 kg N/fed. combined with two or three irrigations gave the best agronomic and quality traits.