Seven true potato seed (TPS) hybrids were evaluated in beds at the International Potato Centre farm (CIP), Kafr El-Zayat, El-Gharbia governorate, Egypt during 2002 and 2003 spring planting seasons, and the resultant seedling tubers were planted during 2002 and 2003 fall planting seasons and during 2003 and 2004 spring planting seasons. This study aimed to produce seed potato free of virus diseases and to reduce the importation of seed tubers from the European countries at expensive prices. The results showed that HPS I/67 hybrid was the best in vegetative characteristics measured as vigor, uniformity and stem length, while HPS I/TPS 13 hybrid showed the lowest values. In addition, HPS I/67, HPS II/67 and Serrana x TPS 67 hybrids gave the highest seedling tuber yield /m²., whereas, the lowest yields /m² were obtained by Atzimba x TPS 13 and Acherrana x TPS 67. The stored seedling tubers that were produced from the seven TPS hybrids in spring season and tubers of Diamant cultivar were used as planting material during fall and spring seasons. Diamant cultivar showed significant increments in uniformity, number of stems and plant height after 45 days from planting. On the other hand, Diamant cultivar recorded the lowest significant values in plant vigor compared with the studied hybrids, but it had the highest mean tuber weight during fall and spring seasons. Highest number of tubers per plant was found in HPS I/67, HPS II/67 and Acherrana x TPS 67 hybrids. Whereas, the highest yield per plot as well as per feddan were recorded by Diamant cultivar and HPS II/67 hybrid in the fall season and by Diamant fallowed by HPS I/67 hybrid in the spring seasons. The highest values of virus infection percentage with PLRV, PVX and PVY were obtained by Diamant and Atzimba x TPS 13, while Serrana x TPS 67 and HPS I/67 hybrids had the lowest virus infection values.