Incidence and distribution of viruses and virus diseases on grapevines (Vitis vinifera.) in different locations in Egypt were determined during March and April 2005 and 2006. Surveys for viruses were carried out at Grapevine areas in which international (imported) and local grapevine (native) cultivars and rootstocks are grown. A total of 446 symptomatic and 1896 a symptomatic leaf samples were collected from 19 different cultivars (native, imported and rootstock as well) were tested by enzyme-linked immunosorbent assay- double antibody Sandwich (DAS-ELISA) for  the most commonly viruses found in grapevine trees:  Grapevine Fanleaf Virus (GFLV), Grapevine Fleck Virus (GFKV), Grapevine Virus A (GVA),Grapevine Leafroll associated Virus -1 (GLRaV-1), Grapevine Leafroll associated Virus -3 (GLRaV-3) , Tomato Ring Spot Virus (ToRSV) and Peach Rosette Mosaic Virus (PeRMV).  GFLV, GFkV, GVA, GLRaV-1, GLRaV-3, ToRSV andPeRMV were found to be widely spread in grapevine propagated material and are considered as economically important grapevine viruses in Egypt. GLRaV-3 was the most widespread virus with (29.5 %) infection followed by GFLV (16 %) infection,GVA (15.9 %), GFKV (13.3%), GLRaV-1 (9.5 %) infection, then the infection rate ofPeRMV 4.2 % in descending order. No viral infection was observed with ToRSV in imported and rootstock cultivars. The infection rate of imported grapevine cultivars and rootstocks were 5.7 % and 0.42 % respectively, while the infection rate of native cultivars was 36.02 %. ‘Romy Ahmer' and  ‘Banaty Abiad', the two major Egyptian cultivars, recoded infection levels of 23.78% and 28.48%, respectively, Bez El-Anza showed 40.42% infection and Siwi Aswad recorded 45.22%.