JRL-23 (Sakha Super 300) and JRL-96 line are new Egyptian Japonica Green Super Rice varieties which reach an optimum yield if fertilized properly and planted in the suitable spacing. A field experiment was conducted in two successive seasons 2019 and 2020 at the Experimental Farm of Rice Research and Training Center (RRTC), Sakha, Kafrelsheikh, Egypt; to evaluate the effect of different levels of fertilizers (N₀P₀K₀, N_82.5P₁₈K₃₀, N_123.75P₂₇K₄₅, N₁₆₅P₃₆K₆₀ and N₃₃₀P₇₂K₁₂₀) and two plant spacing (15×15 cm and 20×20 cm) on the new Egyptian Japonica Green Super Rice varieties Sakha Super 300 and JRL-96 line. The results indicated that the new rice variety Sakha Super 300 was better than the JRL-96 line in all studied characteristics. Moreover, there were significant positive effects of application the N₃₃₀P₇₂K₁₂₀ without any significant differences with N₁₆₅P₃₆K₆₀ under 20×20 cm plant spacing on chlorophyll content, leaf area index, plant height, number of tillers m^-2, panicle weight, panicle length, filled grain weight panicle^-1, number of panicles m^-2, 1000-grain weight, percentage of filled grain, grain and straw yields. N₃₃₀P₇₂K₁₂₀ or N₁₆₅P₃₆K₆₀ showed the best growth characteristics, grain yield and its attributes. Besides, a wider plant spacing of 20×20 cm better than a plant spacing of 15×15 cm in terms of all growth metrics in the two seasons. The new Egyptian Japonica Green Super Rice varieties, Sakha Super 300 and JRL-96 line, were benefiting from the application of N₁₆₅P₃₆K₆₀ with a plant spacing of 20×20 cm, saving NPK dosage up to N₃₃₀P₇₂K₁₂₀ with the maximum grain yield per hectare.