A field experiment was conducted at the Experimental Farm, Faculty of Agriculture, Menoufia University, Shebin El-Kom, Egypt during 2019 and 2020 seasons to study the effect of plant distribution patterns i.e. D1 (sowing cotton in furrows 70 cm width in one ridge with 25 cm between hills and two plants/hill), D2 (sowing cotton in furrows 70 cm width in one ridge with 12.5 cm between hills and one plant/hill), D3 (sowing cotton in beds 140 cm width in two ridges with 25 cm between hills and two plants/hill), D4 (sowing cotton in beds 140 cm width in two ridges with 12.5 cm between hills and one plant/hill) and foliar application with plant growth regulators i.e. control, kinetin (15 ppm), naphthalene acetic acid (15 ppm), mepiquat chloride (100 ppm), mepiquat chloride + kinetin and mepiquat chloride + naphthalene acetic acid at 80 and 95 DAS and on light intensity, morphological traits, flowering and abscission, yield and its components and seed and fiber quality of Egyptian cotton (Giza 86 cv.). The results indicated that sowing cotton plants with D3 pattern surpassed the other plant distribution patterns in most studied characters, while D1 increased plant height but decreased fiber fineness. On the other hand, number of squares/plant, boll weight, 100-seed weight, fiber strength and uniformity index were not significantly affected by plant distribution patterns. Application of growth regulators either single or dual caused a positive effect. NAA or MC+NAA were the superior treatments in most characters studied. However, unsprayed cotton plants increased total abscission. It could be concluded that sowing plants in beds with plant distribution pattern (D3) and foliar application with 100 ppm MC at 80 DAS followed by 15 ppm NAA at 95 DAS was the best interaction treatment in comparison to other interaction treatments to obtain the highest values of light intensity, total dry weight/plant, number of open bolls / plant, seed cotton yield per plant and fed, oil yield /fed and protein yield /fed).