The present study aimed to develop a micropropagation protocol for Cadaman (P. persica x P. davidiana) and Garnem (P. persica x P. dulcis) peach rootstocks. Stem nodal segments with one axillary bud used as explants. MS medium supplemented with different concentrations of BA with or without IBA was studied to determine the best  for multiplication stage,  The results revealed that MS medium supplemented with 2.0 mg/l BA+0.1 mg/l IBA was optimal for shoot proliferation of Cadaman rootstock, whereas MS containing 2.0 mg/l BA+ 0.0 IBA was advisable to Garnem peach rootstock. Concerning the effect of auxin type and concentration on the rooting stage, IBA at 1.0 mg/l was more effective on rooting induction of Cadaman shoots, whereas in case of Garnem rootstock both IBA and NAA auxins had a similar effect on the rooting process at the concentration of 1.0 mg/l individually. After acclimatization, survival rate was 83% and 63.3% for Cadaman and Garmen rootstocks, respectively.