A reliable micropropagation protocol was developed for Red Maple tree for the first time in Iraqi Kurdistan Region to introduce this newly entered species to the local community. At initiation stage, healthy and vigorous cultures were established following a critical disinfestation protocol by bleach (Sodium hypochlorite) at 2.5 % for 20 minutes. The obtained results show that shoots multiplication was successfully enhanced by recording the highest number of shoots and leaves per explant when 0.5 mg.L^-l BA was added to WPM by recording 2.08 shoots/ explant and 13.7 leaves/ explant, respectively. Whereas, the longest shoots were obtained when 1.5 mg.L^-l BA was added by reaching 1.86 cm. For root formation, IBA was more effective than NAA by giving the best rooting parameters. The highest number of roots per explant (3.00 roots/ explant), the longest roots (5.87 cm) and the highest rooting percentage (100%) were obtained when 0.1 mg.L^-l IBA was added to WPM. The half-light intensity (50 feet/ candle) showed better rooting abilities of microshoots than full light intensity conditions (100 feet/ candle) by raising the number of roots from 3.00 to 4.00 roots/ explants, increasing the mean length of roots from 5.87 cm to 6.37 cm when the comparing between the highest rooting parameters at full light illumination. A 100% survival rate was achieved from the acclimatized plantlets in the greenhouse conditions without any abnormal morphological characteristics. It can be recommended that this important plant can be propagated by tissue culture technique toward mass production in the local area.