This investigation was conducted during two successive seasons of 2004 and 2005 at the experimental farm of El-Quassassin Hort. Res. Station, Ismailia Governorate, Egypt, to study the effect of irrigation rates (1610, 3220 or 4876 m3/fed.) and fertilization treatments (NPK and organic fertilizers) on vegetative growth, volatile oil yield and chemical composition of Majorana hortensis L. plant in sandy soil. Application of the high rate of water irrigation (4876 m3/fed./season) gave the highest values of vegetative characteristic i.e., plant height, fresh and dry weight of herb/plant, dry weight of leaves and stems/plant, the yield of fresh and dry weight of herb per plant and per feddan and chemical composition i.e., volatile oil percentage and N, P or K content as compared with 1610 or 3220 m3/fed./season water amount irrigation in the three cuttings during the two seasons. Application NPK fertilization treatment (300 , 300 , 100 kg/fed. as ammonium sulphate, calcium superphosphate and potassium sulphate, respectively) surpassed significantly in vegetative characteristics, oil percentage and chemical content when compared with organic fertilization (compost, poultry and cattle manure). On the other side, poultry manure (10 m3/fed) gave significant increase in the most characteristics under this study when compared to the compost or cattle manure (15 and 20 m3/fed. respectively) fertilization treatments. Irrigation sweet marjoram plants with drip irrigation system at water amount of 4876 m3/fed./season gave 14.305 and 4.688 ton per feddan fresh and dry weight of herb, respectively and 2.63 % (dry weight) volatile oil percentage. The maximum contents of Terpinene-4-Ol and Linalool were obtained from the high level of irrigation (4876 m3/fed/season) combined with cattle manure fertilization treatment. While the high contents of 1,8 Cineole and Limonene were resulted from the high level of irrigation combined with poultry manure fertilization treatment.