Thirty-two sesame landraces (LRs) collected from 13 Governorates were evaluated in Qena and Ismailia during 2005 and 2006 seasons to explore variation within collection of landraces and  to identify superior genotypes.                          The LRs showed variations in days to 50% flowering, plant height, heightof firstcapsule, branches/plant, capsules/plant, seed yield/plant, shelling%, seed index and  seed oil content% for two locations. Variability study showed that, branches/plant, capsules/plant, seed yield/plant and seed index had higher genetic variation than the other traits in Qena . Also, in Ismailia the same traits (except for seed index) beside plant height and shelling% showed the same trend. These results were assured by heritability values in broad sense. Dendrogram of clustering the 32 accessions showed 2 groups (level 85%) which were regrouped at 95% to seven clusters as well as ungrouped LRs in each location. The LRs: No. 2, 21, 27, 30 and  31 and LRs:  No. 6, 10, 11, 15, 27 and 30 were better and could be used directly for developing new improved varieties or for hybridization programs in each Qena and Ismailia, respectively.