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170395

RESPONSE OF CUCUMBER AND OKRA TO COMBINED APPLICATION OF AZOTOBACTER TRANSFORMANTS AND CHEMICAL NITROGEN FERTILIZER

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Last updated: 04 Jan 2025

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Abstract

The present investigation was carried out (Lab. study) in microbial genetics lab., Genetic Dept., Minia University and a field study was carried out at Sakha agriculture research station farm during the two successive summer season of 2004 and 2005.                 DNA extract from Bacillus sp < /em>. (grown under 20% NaCl) was used transform salt isolate to sensitive isolates of Azotobacter vinelandii Wild type. The producing transformants were used to infect cultivars of cucumber (hybrid prince) and Okra Eskandrandi).                 Results showed that the highest frequencies of transformants (330 x 10-6) was obtained on 10% NaCl at 24 hours.                 Results also, indicated that there were significant differences in growth characteristics between the plants inoculated with the tested strains when compared with the control plants (non-inoculated) or the plants inoculated with the wild type strain. All transformants in our study had the best effect on most growth characters and yield as compared with wild type strain.                 The best transformants was T-4 which had the highest percentage of increasing total yield with both crops. The data show that there were differences among both, methods of incubation (Liquid or capsules) in their effects on the two crops in their response. The best method was liquid which had the highest values of all growth characters and total yield.                 Finally, the present investigation recommends the importance of inoculating vegetables crops with transformants of A. vinelandii to improve their growth and yield with using the liquid method.

DOI

10.21608/jpp.2008.170395

Keywords

Okra, cucumber, (Cucumis sativus, L), Azotobacter vinelandii, Azotobacter transformants

Authors

First Name

E.

Last Name

Hassan

MiddleName

A.

Affiliation

Veg. Res. Dept., Hort. Res. Inst. Agric. Centre, Ciza, Egypt,

Email

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City

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Orcid

-

First Name

Manal

Last Name

Abd Allah

MiddleName

A.

Affiliation

Veg. Res. Dept., Hort. Res. Inst. Agric. Centre, Ciza, Egypt,

Email

-

City

-

Orcid

-

First Name

K.

Last Name

Abd Elaziz

MiddleName

A.

Affiliation

Veg. Res. Dept., Hort. Res. Inst. Agric. Centre, Ciza, Egypt,

Email

-

City

-

Orcid

-

First Name

O.

Last Name

Dakhly

MiddleName

F.

Affiliation

Genetic Dept., Fac., of Agric. Minia University

Email

-

City

-

Orcid

-

Volume

33

Article Issue

8

Related Issue

24389

Issue Date

2008-08-01

Receive Date

2021-05-17

Publish Date

2008-08-01

Page Start

5,935

Page End

5,948

Print ISSN

2090-3669

Online ISSN

2090-374X

Article File

JPP_Volume 33_Issue 8_Pages 5935-5948.pdf

PDF . 632.0kB

Link

https://jpp.journals.ekb.eg/article_170395.html

Detail API

https://jpp.journals.ekb.eg/service?article_code=170395

Order

14

Type

Original Article

Type Code

887

Publication Type

Journal

Publication Title

Journal of Plant Production

Publication Link

https://jpp.journals.ekb.eg/

MainTitle

RESPONSE OF CUCUMBER AND OKRA TO COMBINED APPLICATION OF AZOTOBACTER TRANSFORMANTS AND CHEMICAL NITROGEN FERTILIZER

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Article

Created At

22 Jan 2023