Some organic supplements i.e., vermicompost which mixed with mushroom growth substrate (rice straw) at different levels 0.0, 2.5, 5, 10% beside compost teaat concentrate of (1:10) and Azolla plants extract at concentrate of 5% as organic spraying were used to enhance both of biological efficiency and productivity of Pleurotus columbines grown under uncontrolled conditions during the two seasons of 2019 and 2020. The results indicated the ability of vermicompost at high concentrate 10% to encourage mushroom fruiting phases, total yield/bag, biological efficiency, fruit chemical content and all fruit properties except the number of fruit per cluster which appears superior with rice straw sole without any additive (control treatment). On the other side, compost tea as organic spraying was the most influenced to obtain earlier primordial initiation and reduce the days required for both of fruit formation and fruit duration furthermore the high fruit content of protein, carbohydrate, P, K as well as fruit dry matter accumulation. While, Azolla plants extract resulted in the highestfruit characteristic expressed as average number of cluster/bag, weight of cluster/bag, weight of fruit/cluster and weight of basidiocarp but it had a negative effect on number of fruit per cluster. The interaction of vermicompost at rate 10% and Azolla plants extract was the most effective to obtain maximum values of total yield/bag and fungus biological efficiency.