A field experiment designed in randomized complete block design with three replicates was used to evaluate nine promising faba bean lines in F₆ with three cultivars: Giza 716, Giza 843 and Sakha 1 under natural infection of chocolate spot disease at the Agricultural Research Station farm in Etay Al-Baroud during 2018/2019 and 2019/2020. Mean squares due genotypes were highly significant for all studied traits in both seasons as a clear evidence for the wide diversity between these genotypes in their pedigree and these genotypes will differ in their performances for all traits. Line 8, 9 and 3 had highly significant values for most of the traits. All genotypes showed a moderate resistance for chocolate spot except Giza843, Line2 and 4 in both seasons. The genetic variance was the large part in phenotypic variation compared to the environmental variation in all traits and this may be clear that the diversity in all genotypes performances were due the wide genetic diversity of all genotypes. Generally, plant height, 100-seed weight and seed yield/plant had high PCV, GCV and h² values which indicated that this trait is controlled by genetic factor and higher chance to improve this trait through selection. Seed yield/plant, seed yield/plot and seed yield/fad significant negative associated with chocolate spot infection in both seasons. The phenotypic similarity matrix between all genotypes ranged from 12.20 to 118.66. the highest similarity values were showed between Line 8 and Line 6 followed by Giza 843 and Giza 716 and Line 7 and Line 2.