A field experiment was carried out at El-Gemmeiza Agricultural Research Station, El-Gharbia Governorate, Egypt in 2018 season and repeated in 2019 season, to assess the effects of transplanting cotton seedlings of 4 weeks old with their complete root system compared with early and late direct seeding with or without Pix treatments on cotton Giza 86 cultivar productivity. A strip-plot design with four replicates was used. The obtained results indicated that planting with transplanting or early direct seeding significantly increased sympodia, monopodia, earliness attributes, yield components and uniformity index in both seasons, except plant height, internode length, shedding% and first sympodium node which were decreased. Early direct seeding and transplanted cotton significantly increased seed cotton yieldfed^-1 by 16.53 and 11.82%; 17.54 and 12.99% as compared to late direct seeding in the first and second seasons, respectively. Application of Pix at the two levels examined (2.5 cm³/L and 5 cm³/L) significantly decreased growth traits, whereas significantly increased earliness attributes, seed cotton yield, yield components and uniformity index in both seasons, as well as seed index, fiber length and strength in one season only, except days to first flower and shedding% in both seasons which were decreased, whereas first sympodium node did not affected. It could be concluded that early sowing or transplanting seedlings of 4 weeks old and sprayed with Pix three times at the low level (2.5 cm³/L) gave high productivity. That is recommended to overcome the delay of planting and its negative impact under conditions similar to El-Gemmeiza location.