The present investigation was carried out during two successive seasons 2019 and 2020 on Flame seedless grapes cultivar grown in sandy soil at private farm, Naga Hamady, Qena Governorate, Egypt. Eight treatments of mineral N, humic acid, organic and biofertilization applied to study the effect of them on vegetative growth, soil nutrient status, and fruiting of Flame seedless grapevines. The experimental vines were arranged in a complete randomized design with eight treatments and three replications two vine per each. The obtained results could be summarized as follow: Using the recommended dose of nitrogen (RDN) via 25 to 50% mineral plus 25 to 50% Humic acid (HA) or compost and bio-mix significantly increased, leaf area, pruning wood weight, and leaf total chlorophyll as well as leaf nutrient composition compared to use RDN via mineral N fertilizer alone. All combined fertilization treatments significantly increased the yield and improved the cluster and berry traits compared to use RDN via mineral source only. The results of this investigation indicated that, most trace element and soil physical and chemical properties were increased with increasing the level of organic, bio fertilization and humic acid in studied soil. Also, it could be concluded that fertilized vines with 25 to 50% of nitrogen requirements plus HA or compost and bio-mix improved the vegetative growth and nutritional status, as well as, yield, cluster attributes and berry quality of Flame seedless grapevines under  this experiment circumstances.