Barseem clover is the principal forage crop in Egypt, as well as, many other Mediterranean countries. The main objective of the recent study was to trace barseem clover "Trifolium alexandrium, L" landraces with potential resistance to frequent cutting stress. 200 ecotypes of multi- cut barseem clover were collected from ten governorates covering different environments of Egypt. Ecotypes were divided to ten random sets each of 20 ecotypes. Each set was sown in a replicated trial. Depending on data analysis for each set ecotypes, the most tolerance ecotypes to very frequent cutting regime (High green forage yield), were identified during two successive cycles. Commonly, the first cycle of selection (C₁) for frequent-cutting tolerance gave a cumulative realized gain over cuttings in green forage yield over the base population reached 9.09, 76.38 and 46.62% when evaluated under infrequent, frequent and very frequent cutting regimes, respectively. Meanwhile, the second cycle of selection (C₂) for frequent cutting tolerance gave a substantial realized gain in green forage yield relative to cycle one reached 108.6, 94.52 and 180.56% when evaluated under infrequent, frequent and very frequent cutting. Cumulative realized gain over cuttings in green forage yield related to the average of the evaluated check varieties were -23.14, 104.2 and 177.6 when evaluated under infrequent, frequent and very frequent cutting regimes. Meanwhile, the second cycle of selection (C₂) gave a cumulative realized gain over cuttings in green forage yield relative to the average of the first cycle (C₁) reached 67.32, 218.6 and 367.1% when evaluated under infrequent, frequent and very frequent cutting regimes. It was evident that the realized gain in green forage yield from selection for frequent cutting tolerance among barseem ecotypes was more obvious after the second cycle of selection proposing that successive cycles of selection might result in better gains. Also, the superiority of selected cycles (C₁ and C₂) was clearly shown over the studied check varieties. Meanwhile, the advantage of selection for frequent cutting tolerance was sounder and more illustrative, when improved cycles were evaluated under very frequent cutting regime.