In 2015/2016 and 2017/2018 Two experiments were carried out at Sakha Agricultural Research Station, to investigate the response of wheat crop to nitrogen fertilization levels(0, 25, 50 and 75 kg N/fed)on growth, chemical composition and productivity of six wheat genotypes(Misr 1, Sakha 95, Giza 171, Shandaweel 1, Misr 2 and promising line developed from Sakha breeding program(SPL2015).This research conducted using the split-plot design with four replications. In which, the main-plots and sub-plots assigned to levels of nitrogen fertilization and wheat genotypes, respectively. Results showed that high levels of nitrogen levels(75 kg N/fed)gave the highest values of crop growth rate, flag leaf area, photosynthetic pigments and Nitrogen percentage in grains. Moreover, increasing nitrogen fertilizer increased yield and its components significantly. Sakha 95 cultivar produced the highest values of all studied characteristics in both growing seasons. Meanwhile, the cultivar Misr 2 showed the highest number of spikelets/spike, additionally recorded the second best results after Sakha 95.On the other hand, Shandaweel 1 cultivar gave the longest spikes; Giza 171 cultivar recorded the highest values of 1000-grain weight,Misr1 gave the highest N% content for the grain.  However, Sakha 95 under 50 kg N/fed ranked secondly in most treatments without significant differences between Sakha 95 under 75 kg N level /fed for both seasons. It can be concluded that Sakha 95 had the highest nitrogen use efficiency and this reflecting in reduction of  production costs and environmental pollution by saving 25 kg N/fed under such environmental conditions of Sakha district, Kafr El-Sheikh Governorate, Egypt.