Leaves of Green nightshade (Solanum viride Solander ex Forst. f.) family: Solanaceae were used as source of explants to start micropropagation. Leaves were successfully sterilized using the treatment of sodium hypochlorite (NaOCl) at 1.0% that showed the highest percentage of survival without any contamination. The highest fresh weight of callus/explant was recorded when the leaf segments were cultured on MS media contained 0.5 mg/l BA and 1.5 mg/l NAA. Interestingly, some treatments recorded direct organogenesis (direct shoots and roots) from the leaf explants. The highest direct shoot formation was recorded with MS media supplemented with 2 mg/l BA alone. However, it was clear that all shoots obtained with the responded treatments were vitrified. Therefore, in a trial to obtain unvitrified shoots as indirect organogenesis through callus formation and differentiation, all indirect obtained shoots were vitrified except some shoots (4.7 % of them) obtained on MS media supplemented with 1.0 mg/l BA alone. These unvitrified shoots were successfully acclimatized in soil mixture of sand and peatmoss (1:2, v: v) that showed the best growth and percentage of survival (95%).