The banding patterns of malate dehydrogenase (EC1.1.1.37), glycerol dehydrogenase (EC1.1.1.72), peroxidase (EC1.11.1.7), esterase (EC3.1.1.1), and acid phosphatase (EC3.1.3.2) were determined by polyacrylamide gel electrophoresis for 28 isolates of Macrophomina phaseolina. A total of 96 phenotypes was detected for all the enzymes. Esterase gave the greatest number of polymorphic banding patterns. Cluster analysis of the isozyme banding patterns by the unweighted pair-group method based on arithmetic means placed the isolates in several groups; however, geographic origin of the isolates or source (host) did not exhibit clear correlation with the isozyme electrophoretic grouping results.