Different chemical inducers namely, DL-β-aminobutyric (βABA), salicylic acid (SA) and indole acetic acid (IAA) were used to induce resistance in wheat plants against leaf rust disease caused by Puccinia triticina Eriks under greenhouse conditions. Seedlings were sprayed by different concentrations of inducers at the second leaf stage (14 days old) and challenge inoculation by uredia was carried out at three leaf stage (19 days old). All concentrations of the tested compound led to significant reduction in infection type and mean number of pustules on both tested wheat varieties i.e. resistant (Giza-168), and susceptible (Sids-1). DL-β-aminobutyric acid at 1000μg/ml gave the best results in controlling leaf rust followed by the higher concentration of indole acetic acid and salicylic acid in descending order. All chemical inducers led to an increase of free phenols in both tested cultivars, but conjugated phenols were increased in the susceptible cv. (Sids-1) and decreased in resistance one (Giza-168).Wheat seedlings treated with βABA or IAA, and then challenge inoculation with Puccinia triticina, showed higher level of peroxidase activity. Meanwhile, the level of peroxidase in the resistant cultivar Giza-168 was higher in seedlings treated with IAA followed by βABA and SA. Cross sections of treated wheat leaves showed higher rate of lignification in the cell walls of xylem vessels due to βABA application as compared to non-treated leaves. Data revealed that rust incidence was reduced at site of application (2^nd leaf), as well as at remote site (3^rd leaf). These results proved that these compounds could induce systemic acquired resistance in wheat plants against P.triticina.