Basil plant is one of the most popular medicinally herbs. Vascular wilt disease caused by Fusarium oxysporum is considering one of the most important diseases on basil in Egypt. Fusarium oxysporum isolates were isolated from all diseased plants showing symptoms of leaf yellowing, stunting, wilted foliage and root rots. All tested isolates of the fungus were found to be pathogenic for basil plants   with different degrees. Pathogenicity test with representative isolates indicated that the causal agent is F. oxysporum that causes damping off, wilt, crown and root rot on basil seedlings and plants, where, isolate No.(2) showed more virulent. Under greenhouse conditions and artificial infestation, basil cultivars were differed in their susceptibility to infection by the fungus.Double flowers basil cv. showed more resistant for the disease symptoms, followed by Red leaves cv. Two techniques, vegetative or mycelial compatibility grouping and SDS (PAGE) fingerprinting, were showed differences between Fusarium isolates. Bio-Zeid bioproducts was more effective against Fusarium disease incidence. Also, seed dressing by combining of Vitavax-200 and Rizolex-T showed significant effects in controlling the disease incidence and produced the highest value of healthy plants.